|Title||Use of SPIDER and SPIRE in Image Reconstruction.|
|Publication Type||Journal Article|
|Year of Publication||2012|
|Authors||Leith, AD, Baxter, B, Frank, J|
|Journal||International Tables for Crystallography|
|Issue||Crystallography of Biological Macromolecules|
SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.