Use of SPIDER and SPIRE in Image Reconstruction.

TitleUse of SPIDER and SPIRE in Image Reconstruction.
Publication TypeJournal Article
Year of Publication2012
AuthorsLeith, AD, Baxter, B, Frank, J
JournalInternational Tables for Crystallography
IssueCrystallography of Biological Macromolecules

SPIDER is a comprehensive command-operated suite of programs for image processing in electron microscopy, with special emphasis on single-particle reconstruction. This chapter provides an outline of the way SPIDER and SPIRE, its interactive graphical user interface, may be used to obtain a three-dimensional reconstruction from projections of many randomly oriented realizations of a biological molecule.


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