TY - JOUR T1 - SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs. JF - Nat Protoc Y1 - 2008 A1 - Shaikh, Tanvir R A1 - Gao, Haixiao A1 - Baxter, Bill A1 - Asturias, Francisco J A1 - Boisset, Nicolas A1 - Leith, ArDean A1 - Frank, Joachim KW - Image Processing, Computer-Assisted KW - Microscopy, Electron KW - Models, Molecular KW - Molecular Structure KW - Software KW - User-Computer Interface AB -

This protocol describes the reconstruction of biological molecules from the electron micrographs of single particles. Computation here is performed using the image-processing software SPIDER and can be managed using a graphical user interface, termed the SPIDER Reconstruction Engine. Two approaches are described to obtain an initial reconstruction: random-conical tilt and common lines. Once an existing model is available, reference-based alignment can be used, a procedure that can be iterated. Also described is supervised classification, a method to look for homogeneous subsets when multiple known conformations of the molecule may coexist.

VL - 3 UR - http://www.ncbi.nlm.nih.gov/pubmed/19180078 IS - 12 ER -