02450nas a2200397   4500008004100000022001400041245010000055210006900155260001200224300001000236490000700246520132400253653001201577653002701589653002401616653001601640653001301656653003101669653001601700653001601716653000901732653001801741653001601759653000901775653002301784653003001807653000801837100001701845700002301862700001901885700002001904700001801924700001901942700001901961856007201980       2005                        eng d  a0168-827800aPathways for the regulation of body iron homeostasis in response to experimental iron overload.0 aPathways for the regulation of body iron homeostasis in response  c10/2005  a711-90 v433 aBACKGROUND/AIMS:
Secondary iron overload is a frequent clinical condition found in association with multiple blood transfusions.
METHODS:
To gain insight into adaptive changes in the expression of iron genes in duodenum, liver and spleen upon experimental iron overload we studied C57BL/6 mice receiving repetitive daily injections of iron-dextran for up to 5 days.
RESULTS:
Iron initially accumulated in spleen macrophages but with subsequent increase in macrophage ferroportin and ferritin expression its content in the spleen decreased while a progressive storage of iron occurred within hepatocytes which was paralleled by a significant increase in hepcidin and hemojuvelin expression. Under these conditions, iron was still absorbed from the duodenal lumen as divalent metal transporter-1 expressions were high, however, most of the absorbed iron was incorporated into duodenal ferritin, while ferroportin expression drastically decreased and iron transfer to the circulation was reduced.
CONCLUSIONS:
Experimental iron overload results in iron accumulation in macrophages and later in hepatocytes. In parallel, the transfer of iron from the gut to the circulation is diminished which may be referred to interference of hepcidin with ferroportin mediated iron export, thus preventing body iron accumulation.10aAnimals10aDisease Models, Animal10aDisease Progression10aDNA Primers10aDuodenum10aGene Expression Regulation10aHepatocytes10aHomeostasis10aIron10aIron Overload10aMacrophages10aMice10aMice, Inbred C57BL10aPolymerase Chain Reaction10aRNA1 aTheurl, Igor1 aLudwiczek, Susanne1 aEller, Philipp1 aSeifert, Markus1 aArtner, Erika1 aBrunner, Peter1 aWeiss, Günter  uhttp://www.sciencedirect.com/science/article/pii/S0168827805003168#