01443nas a2200205 4500008004100000022001400041245010400055210006900159260001200228300001100240490000800251520078400259653002801043653004001071100001701111700002501128700001701153700001901170856004801189 2009 eng d a1095-865700aDetermination of signal-to-noise ratios and spectral SNRs in cryo-EM low-dose imaging of molecules.0 aDetermination of signaltonoise ratios and spectral SNRs in cryoE c05/2009 a126-320 v1663 a

Attempts to develop efficient classification approaches to the problem of heterogeneity in single-particle reconstruction of macromolecules require phantom data with realistic noise models. We have estimated the signal-to-noise ratios and spectral signal-to-noise ratios for three steps in the electron microscopic image formation from data obtained experimentally. An important result is that structural noise, i.e., the irreproducible component of the object prior to image formation, is substantial, and of the same order of magnitude as the reproducible signal. Based on this result, the noise modeling for testing new classification techniques can be improved.

10aCryoelectron Microscopy10aImage Processing, Computer-Assisted1 aBaxter, Bill1 aGrassucci, Robert, A1 aGao, Haixiao1 aFrank, Joachim uhttp://www.ncbi.nlm.nih.gov/pubmed/19269332