Determination of signal-to-noise ratios and spectral SNRs in cryo-EM low-dose imaging of molecules.

TitleDetermination of signal-to-noise ratios and spectral SNRs in cryo-EM low-dose imaging of molecules.
Publication TypeJournal Article
Year of Publication2009
AuthorsBaxter, B, Grassucci, RA, Gao, H, Frank, J
JournalJ Struct Biol
Volume166
Issue2
Pagination126-32
Date Published05/2009
ISSN1095-8657
KeywordsCryoelectron Microscopy, Image Processing, Computer-Assisted
Abstract

Attempts to develop efficient classification approaches to the problem of heterogeneity in single-particle reconstruction of macromolecules require phantom data with realistic noise models. We have estimated the signal-to-noise ratios and spectral signal-to-noise ratios for three steps in the electron microscopic image formation from data obtained experimentally. An important result is that structural noise, i.e., the irreproducible component of the object prior to image formation, is substantial, and of the same order of magnitude as the reproducible signal. Based on this result, the noise modeling for testing new classification techniques can be improved.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/19269332
DOI10.1016/j.jsb.2009.02.012
Alternate JournalJ. Struct. Biol.
PubMed ID19269332
PubMed Central IDPMC2700974

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